Prion protein N1 cleavage peptides stimulate microglial interaction with surrounding cells.

摘要: Microglia act as the protective immune cell of brain. By surveying tissue to identify and rectify problems, they function maintain health brain cells. The prion protein N-terminal cleavage fragment, N1, has demonstrated neuroprotective activities in vitro vivo. This study aimed elucidate whether N1 could modulate microglial and, if so, determine consequences for surrounding tissue. Using a mixed neuronal lineage microglia co-culture system, we showed that stimulation changed overall morphology metabolism, suggesting enhanced cellular viability. Furthermore, induced an increase Cxcl10 secretion co-cultures. Recombinant Cxcl10, administered exogenously, mediated changes culture metabolism absence microglia, but no effect was observed on cultured their own. Direct cell-to-cell contact required influence co-cultures, this linked with restructuring membrane composition include higher GM1 content at interaction sites Our findings show can play regulatory role context inter-connected network cells by changing both cytokine secretion.