Inactivation of poliovirus type 1 in mixed human and swine wastes and by bacteria from swine manure.

摘要: The persistence of poliovirus type 1 (PO1) in mixed septic tank effluent and swine manure slurry was determined, the antiviral effects several bacterial cultures isolated from were demonstrated. In two field experiments, PO1 consistently inactivated more rapidly waste than control Dulbecco's phosphate-buffered saline (D-PBS). D values (time [in days] for a 90% reduction virus titer) 18.7 29.9 56.5 51.8 D-PBS control, respectively. inactivation temperature dependent. A comparison raw waste, autoclaved bacterium-free filtrate at same pH temperatures provided an initial demonstration that is related, least part, to microbial activity. At 25 degrees C, value 6.8 11.2 10.5 waste. 37 1.3, 3.9, 3.1 these three suspending media, Three isolates which had shown screening test each caused effect other microorganisms excluded. Inhibition by protease inhibitors suggests due part proteolytic enzymes produced bacteria