Isolation and Purification of Bioactive Proteins from Bovine Colostrum

摘要: Bovine colostrum is the milk secreted by cows during first few days after parturition. It contains many essential nutrients and bioactive components, including growth factors, immunoglobulins (Igs), lactoperoxidase (Lp), lysozyme (Lys), lactoferrin (Lf), cytokines, nucleosides, vitamins, peptides oligosaccharides, which are of increasing relevance to human health. Much research work has been done on structure function bovine proteins. IgG was widely utilised in immunological supplementation foods, specifically infant formulate, yielded sales approximately US$100 million 2007 (Gapper, et al., 2007). In highly competitive valuable international market for IgG-containing products, some products usually priced based content. Another important protein from lactoferrin. Its diverse range biological activities such as anti-infective toward a broad spectrum species, antioxidant promotion iron transfer expanding demand market. also exhibits potential chemoprevention colon other cancers natural gradient. Apart two kinds proteins, ┙-lactalbumin claimed an food additive formula due its high content tryptophan protective against ethanol stress-induced gastric mucosal injury. ┚Lactoglobulin commonly used stabilize emulsions surface-active properties. serum albumin (BSA) gelation properties it interest number therapeutic applications (Almecija, Therefore, fractionation recovery isolation these proteins great scientific commercial interest. As result this use there establishing more efficient, robust low cost processes purify them. Although deals studies have separation purification their wide application industry, medicine supplements, large scale production system downstream processing recombinant antibodies still represents major issue. Lu (Lu, 2007) designed two-step ultrafiltration process followed fast flow strong cation exchange chromatography isolate LF scale. A stepwise procedure crude conducted using preparative-scale