Doxorubicin Affects Expression of Proteins of Neuronal Pathways in MCF-7 Breast Cancer Cells

作者: Peter Kruzliak , Jan Sabo , Manfred Heller , Marian Petrovic , Marian Petrovic

DOI: 10.7892/BORIS.80764

关键词:

摘要: In the present article, we report on semi-quantitative proteome analysis and related changes in protein expression of MCF-7 breast cancer cell line following treatment with doxorubicin, using precursor acquisition independent from ion count (PAcIFIC) mass spectrometry method. PAcIFIC represents a cost-effective easy-to-use proteomics approach, enabling for deep sequencing minimal sample handling. The acquired proteomic data sets were searched regulated Reactome pathways Gene Ontology annotation terms new algorithm (SetRank). Using this identified significant (≤0.05), such as chromatin organization, DNA binding, embryo development, condensed chromosome, sequence-specific response to oxidative stress toxin, well others. These are already well-described being susceptible chemotherapeutic drugs. Additionally, found neuron central nervous system differentiation, projection membrane SNAP receptor activity. later might indicate biological mechanisms molecular level causing known side-effect doxorubicin chemotherapy, characterized cognitive impairment, also called 'chemo brain'. Mass available via ProteomeXchange identifier PXD002998.

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