Effect of acidic conditioners on dentin morphology, molecular composition and collagen conformation in situ.

摘要: Objective: The aim of the study was to evaluate effect some acidic conditioners on dentin morphology, molecular composition and collagen conformation in situ. Methods: Smear layer-covered specimens prepared from third molars immediately after extraction were imaged by tapping mode AFM analyzed MIR-FTIR spectroscopy. same subjected conditioning treatments with CA Agent (Kuraray), Scotchbond Etchant (3M Dental Products) MP gels then again. extent decalcification at uppermost 2 μm region calculated percentage reduction -PO4/amide I peak area ratio conditioned relative their individual smear references. These results compared ANOVA Scheffe´statistical analyses. conformational changes type studied qualitatively deconvoluting amide bands spectra assigning band components carbonyl hydrogen bonding states related α-helix structure. Results: All removed layer, funneled tubules, increased intertubular roughness contaminated surfaces residues irreversibly adsorbed thickening agents. Conditioned showed a orthophosphates carbonates an increase I, II III groups. manifested significantly lower than etchants (p < 0.05). Collagen involved decrease intermolecular bonded carbonyls associated structure enhancement imide water, which suggest denaturation. Significance: Apart decalcification, induced considerable mostly denaturation processes. In addition, residual thickeners substantially modified morphology surfaces. findings show complex interaction pathways between great potential modern situ imaging analysis techniques probing these interactions.