Chlorhexidine binding to mineralized versus demineralized dentin powder

摘要: Objectives The purposes of this work were to quantitate the affinity and binding capacity chlorhexidine (CHX) digluconate mineralized versus demineralized dentin powder determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods Dentin was made coronal extracted human third molars. Standard amounts tumbled increasing concentrations CHX (0–30 mM) for 30 min at 37 °C. After centrifuging tubes, supernatant removed decrease concentration quantitated by UV-spectroscopy. CHX-treated resuspended one four solutions 3 min. The amount debound solvents also UV-spectroscopy. Results As medium increased, increased up 6.8 μmol/g dry powder. Demineralized took significantly (p < 0.01) more CHX, reaching 30.1 μmol CHX/g Debinding order: HEMA < ethanol < 0.05 M NaCl < water. highest occurred 30 mM (1.5 wt.%). Significance As is not HEMA, it may remain bound during resin–dentin bonding. This be responsible long-term efficacy as an MMP inhibitor bonds.