Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes

摘要: The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs known. Here, we screened 3,080 specimens from 54 bat species representing 11 families for hepadnaviral DNA. Ten (0.3%) Panama Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative orthohepadnavirus based on lengths (3,149–3,377 nt), presence middle surface X-protein genes, sequence distance criteria. Hepatic tropism bats was shown by quantitative PCR situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected all viruses cross-reacted sera against the core protein, concordant phylogenetic relatedness these One Uroderma bilobatum, tent-making bat, monoclonal antibodies antigenicity determining S domain. Up 18.4% contained hepadnaviruses. Infectious clones were generated study detail. Hepatitis D particles pseudotyped proteins U. bilobatum HBV, but neither other two could infect primary Tupaia belangeri hepatocytes. Hepatocyte infection occurred through receptor sodium taurocholate cotransporting polypeptide not be neutralized vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication Our data suggest that may have been ancestral sources primate observed potential might affect concepts aimed at eradicating