14C2H4: Its Incorporation and Metabolism by Pea Seedlings under Aseptic Conditions

摘要: The effects of various treatments on the recently reported system in pea ( Pisum sativum cv. Alaska), which results a ) incorporation 14 C 2 H 4 into tissue and b conversion to CO , was investigated using 2-day-old etiolated seedlings exhibit maximum response. Heat treatment (80 C, 1 min) completely inhibited both whereas homogenization but only partially . Detaching cotyledons from root-shoot axis immediately before exposing detached together with markedly reduced Increasing concentration 0.14 over 100 μl/l progressively increased rate being greater than below 0.3 Reduction O 99% inhibition occurring under anaerobic conditions. addition (5%) severely without significantly affecting incorporation. Exposure fluorescent light during effect. Similarly, indoleacetic acid, gibberellic benzyladenine, abscisic dibutyryl cyclic adenosine monophosphate had no significant effect either or possibilities that tissues its is linked ethylene action, it represents means reducing endogenous level, are discussed. Several problems encountered use polyethylene vials, rubber serum stoppers, Clorox, microbial contamination also described.