E2F7 regulates transcription and maturation of multiple microRNAs to restrain cell proliferation

摘要: E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora target genes, including those coding for microRNAs (miRNAs), control cell cycle progression. Recent work has described atypical factor E2F7 as transcriptional repressor cycle-related protein-coding genes. However, contribution miRNA gene during not been defined. We have performed genome-wide RNA sequencing analysis identify E2F7-regulated miRNAs and show that plays major role in negative regulation set promote cellular proliferation. provide mechanistic evidence an interplay between canonical E2F1-3 multiple miRNAs. miR-25, -26a, -27b, -92a -7 is controlled at level by antagonistic activity E2F1-3. By contrast, let-7 indirectly through novel E2F/c-MYC/LIN28B axis, whereby modulate c-MYC LIN28B levels impact processing maturation. Taken together, our data uncover new regulatory network involving post-transcriptional mechanisms restrain progression repression proliferation-promoting