Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair
作者： Emmanuele Crespan , Antonia Furrer , Marcel Rösinger , Federica Bertoletti , Elisa Mentegari
DOI: 10.1038/NCOMMS10805
关键词:
摘要: Oxidative stress is a very frequent source of DNA damage. Many cellular polymerases (Pols) can incorporate ribonucleotides (rNMPs) during synthesis. However, whether oxidative stress-triggered repair synthesis contributes to genomic rNMPs incorporation so far not fully understood. Human specialized Pols β and λ are the important enzymes involved in tolerance, acting both base excision translesion past lesion 7,8-dihydro-8-oxoguanine (8-oxo-G). We found that Pol β, greater extent than opposite normal bases or 8-oxo-G, with different fidelity. Further, 8-oxo-G delays by glycosylases. Studies β- λ-deficient cell extracts suggest levels greatly affect rNMP lesions.
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