Orientation of dye molecules in DNA-based films with chain alignment and judgment of their DNA-binding modes.

摘要: Novel composite films of chain-oriented DNA, which contain the DNA-binding dyes aligned in specific orientation, were successfully prepared by drying solution under a horizontal magnetic field. Most dye-DNA showed linear dichroism, as revealed polarized ultraviolet-visible (UV-vis) spectroscopy. The intercalators, ethidium bromide and acridine orange, fixed DNA similar binding manner solutions. Also, Hoechst 33258 4',6-diamidino-2-phenylindole found to be along minor groove, even solid films. Thus, our new method preparing with chain orientation is useful for aligning small molecules, it will provide views novel anisotropic materials expected various application fields. We used this prepare newly designed original compounds. Seven nine judged bind obviously intercalators UV-vis manners further analyzed fluorescence anisotropy measurements. On basis curves rotational angle dependence anisotropy, we able estimate angles between transition-dipole moments axis DNA. Interestingly, two compounds tilted forms regard plane base pairs. emphasize here that using very convenient identifying modes double-stranded