How to protect human pancreatic enzyme activities in frozen duodenal juice

摘要: Abstract We determined whether activity of pancreatic enzymes could be maintained in frozen duodenal juice by diluting the specimens or adding nutrients a chymotrypsin inhibitor. Human was obtained during cholecystokinin octapeptide IV administration. Trypsin, chymotrypsin, lipolytic, lipase, and colipase activities were measured fresh undiluted diluted (1:4 1:16 with saline T-tube bile) as well after CaCl 2 , casein, triolein, Subsequently, samples at −20 °C, enzyme 1, 2, 3, 7, 14, 28, 56 days. Activities did not change freezer storage. Trypsin survival variable from different subjects. By contrast, to which no nutrient only had been added, 90%, 65%, 40% ( P = 0.05 vs. undiluted) lipolytic lost days 1:4 samples, respectively. The loss prevented r 0.97) but weakly associated 0.49). In summary, are preserved can used accurately assess concentrations thawing samples. If it is necessary measure freezing lipase casein inhibitor before freezing.