Brain death-related energetic failure of the donor heart becomes apparent only During storage and reperfusion : An ex vivo phosphorus-31 magnetic resonance spectroscopy study on the feline heart

摘要: Background and Objective: Recently, we have shown, by using localized in vivo phosphorus-31 magnetic resonance spectroscopy (31P MRS) of the anterior left ventricular wall, that brain death (BD) is not associated with reduced myocardial energy status. In this study, applied ex 31P MRS entire heart to study effects BD on status feline donor following explantation. Methods: We used cats (6 6 controls [C]) a 26-hour protocol. After 2 hours preparation, induced filling an intracranial balloon at t = 0 hour. At hours, hearts were arrested St. Thomas’ Hospital cardioplegic solution, explanted, stored same solution 4°C 4.7 Tesla magnet for 17 hours. Subsequently, reperfused Langendorff mode 38°C 1 The first 5-minute spectrum was obtained hour after crossclamping aorta; subsequent spectra every during storage 5 minutes reperfusion. end, dried weighed. Phosphocreatine (PCr), γ-adenosine triphosphate (γ-ATP), inorganic phosphate (Pi), phosphomonoesters (PME), expressed per g dry weight. intracellular pH (pHi) PCr/ATP ratio calculated. Results: During storage, identified significant but similar decrease pHi, ratio, PCr both groups. reperfusion, pHi recovered similarly groups, whereas recovery group significantly lower (p < 0.05). Pi PME increased groups lesser extent This difference disappeared γ-ATP already onset remained so throughout reperfusion 0.05 vs C). Contractile capacity lost all hearts, except group. Conclusion: Brain death-related failure energetic integrity becomes apparent only when ischemic preservation