作者: L Bowie , F Esters , J Bolin , N Gochman
DOI: 10.1093/CLINCHEM/22.4.449
关键词:
摘要: We describe a new technique for monitoring intra-cuvette temperatures in temperature-controlled spectrophotometric cells by use of two solutions, one which absorbance is highly sensitive to temperature and insensitive temperature. The first solution, cresol red tris(hydroxymethyl)aminomethane buffer, used construct an absorbance-vs.-temperature calibration curvae. second sodium hydroxide, optical the system be tested. combination these procedures allows accurate estimation show how this applied measurement several types laboratory instrumentation.