Surface functionalization of quantum dots with fine-structured pH-sensitive phospholipid polymer chains.

作者: Yihua Liu , Yuuki Inoue , Kazuhiko Ishihara

DOI: 10.1016/J.COLSURFB.2015.08.001

关键词:

摘要: To add novel functionality to quantum dots (QDs), we synthesized water-soluble and pH-responsive block-type polymers by reversible addition-fragmentation chain transfer (RAFT) polymerization. The were composed of cytocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer segments, which contain a small fraction active ester groups can be used conjugate biologically compounds the polymer, poly(2-(N,N-diethylamino) ethyl methacrylate (DEAEMA)) segments. One terminal had hydrophobic alkyl group that originated from RAFT initiator. This bind layer on QD surface. A fluorescent dye was conjugated chains via group. have an amphiphilic nature in aqueous medium. thus easily bound surface upon evaporation solvent solution containing QDs, yielding QD/fluorescence dye-conjugated hybrid nanoparticles. Fluorescence resonance energy (FRET) between QDs (donors) molecules (acceptors) obtain information conformational dynamics immobilized polymers. Higher FRET efficiency QD/fluorescent nanoparticles observed at pH 7.4 as compared 5.0 due stretching-shrinking motion poly(DEAEMA) segments response changes pH. We concluded MPC polymer-modified could evaluate cells fluorescence based cytocompatibility polymer.

参考文章(55)
Kazuhiko Ishihara, Yuriko Tsukamoto, Yusuke Goto, Yuuki Inoue, Enhanced and Specific Internalization of Polymeric Nanoparticles to Cells IFMBE Proceedings. pp. 262- 265 ,(2013) , 10.1007/978-3-642-32183-2_66
Mingyong Han, Xiaohu Gao, Jack Z. Su, Shuming Nie, Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules Nature Biotechnology. ,vol. 19, pp. 631- 635 ,(2001) , 10.1038/90228
Peter Mitchell, Turning the spotlight on cellular imaging. Nature Biotechnology. ,vol. 19, pp. 1013- 1017 ,(2001) , 10.1038/NBT1101-1013
Kenichi Fukuda, Ryusuke Enomoto, Kazuhiko Ishihara, Yotaro Morishima, Shin-ichi Yusa, Thermo-Responsive and Biocompatible Diblock Copolymers Prepared via Reversible Addition-Fragmentation Chain Transfer (RAFT) Radical Polymerization Polymers. ,vol. 6, pp. 846- 859 ,(2014) , 10.3390/POLYM6030846
Oliver I. Strube, Lars Nothdurft, Zaure Abisheva, Gudrun Schmidt-Naake, New Functional Block Copolymers via RAFT Polymerization and Polymer-Analogous Reaction Macromolecular Chemistry and Physics. ,vol. 213, pp. 1274- 1284 ,(2012) , 10.1002/MACP.201200026
Kok Hou Wong, Thomas P Davis, Christopher Barner-Kowollik, Martina H Stenzel, None, Honeycomb structured porous films from amphiphilic block copolymers prepared via RAFT polymerization Polymer. ,vol. 48, pp. 4950- 4965 ,(2007) , 10.1016/J.POLYMER.2007.06.048
Kazuhiko Ishihara, Tsuyoshi Tsuji, Toshikazu Kurosaki, Nobuo Nakabayashi, Hemocompatibility on graft copolymers composed of poly(2‐methacryloyloxyethyl phosphorylcholine) side chain and poly(n‐butyl methacrylate) backbone Journal of Biomedical Materials Research. ,vol. 28, pp. 225- 232 ,(1994) , 10.1002/JBM.820280213
Shin-ichi Yusa, Kenichi Fukuda, Tohei Yamamoto, Kazuhiko Ishihara, Yotaro Morishima, Synthesis of well-defined amphiphilic block copolymers having phospholipid polymer sequences as a novel biocompatible polymer micelle reagent. Biomacromolecules. ,vol. 6, pp. 663- 670 ,(2005) , 10.1021/BM0495553
Xingyong Wu, Hongjian Liu, Jianquan Liu, Kari N. Haley, Joseph A. Treadway, J Peter Larson, Nianfeng Ge, Frank Peale, Marcel P. Bruchez, Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots Nature Biotechnology. ,vol. 21, pp. 41- 46 ,(2003) , 10.1038/NBT764
Ryosuke Matsuno, Kazuhiko Ishihara, Molecular-Integrated Phospholipid Polymer Nanoparticles with Highly Biofunctionality Macromolecular Symposia. ,vol. 279, pp. 125- 131 ,(2009) , 10.1002/MASY.200950519