作者: Yihua Liu , Yuuki Inoue , Kazuhiko Ishihara
DOI: 10.1016/J.COLSURFB.2015.08.001
关键词:
摘要: To add novel functionality to quantum dots (QDs), we synthesized water-soluble and pH-responsive block-type polymers by reversible addition-fragmentation chain transfer (RAFT) polymerization. The were composed of cytocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer segments, which contain a small fraction active ester groups can be used conjugate biologically compounds the polymer, poly(2-(N,N-diethylamino) ethyl methacrylate (DEAEMA)) segments. One terminal had hydrophobic alkyl group that originated from RAFT initiator. This bind layer on QD surface. A fluorescent dye was conjugated chains via group. have an amphiphilic nature in aqueous medium. thus easily bound surface upon evaporation solvent solution containing QDs, yielding QD/fluorescence dye-conjugated hybrid nanoparticles. Fluorescence resonance energy (FRET) between QDs (donors) molecules (acceptors) obtain information conformational dynamics immobilized polymers. Higher FRET efficiency QD/fluorescent nanoparticles observed at pH 7.4 as compared 5.0 due stretching-shrinking motion poly(DEAEMA) segments response changes pH. We concluded MPC polymer-modified could evaluate cells fluorescence based cytocompatibility polymer.