Comprehensive Quantification of Triacylglycerols in Soybean Seeds by Electrospray Ionization Mass Spectrometry with Multiple Neutral Loss Scans

摘要: Soybean seeds are an important source of vegetable oil and biomaterials. The content individual triacylglycerol species (TAG) in soybean is difficult to quantify accurate rapid way. present study establishes approach TAG utilizing electrospray ionization tandem mass spectrometry with multiple neutral loss scans. Ten scans were performed detect the fatty acyl chains TAG, including palmitic (P, 16:0), linolenic (Ln, 18:3), linoleic (L, 18:2), oleic (O, 18:1), stearic (S, 18:0), eicosadienoic (20:2), gadoleic (20:1), arachidic (20:0), erucic (22:1), behenic (22:0). abundance ten at 46 masses (mass-to-charge ratio, m/z) determined after isotopic deconvolution correction by adjustment factors each mass. direct sample infusion internal standards allowed a quantification species. Ninety-three resolved their levels determined. most abundant LLL, OLL, LLLn, PLL, OLLn, OOL, POL, SLL. Many new detected quantified. This shotgun lipidomics should facilitate metabolism genetic breeding for desirable composition.