Development of new tools for studying gene function in fungi based on the Gateway system
作者： Hadas Shafran , Itay Miyara , Ravit Eshed , Dov Prusky , Amir Sherman
DOI: 10.1016/J.FGB.2008.04.011
关键词:
摘要: Genomic information of many fungi has been released but large scale functional genomic studies are still limited by a lack high-throughput methods. The low rates homologous recombination and transformation limiting steps in filamentous fungi, the molecular tools also lagging behind. In this paper we describe two new for that based on Gateway technology. One system is RNAi vector allows gene silencing manner. other deletion construct system. These systems were tested using PAC1 Colletotrichum gloeosporioides. Using these types approaches, genomics experiments can be performed fungi.
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Sigal Horowitz, Stanley Freeman, Aida Zveibil, Oded Yarden, None, A defect in nir1, a nirA-like transcription factor, confers morphological abnormalities and loss of pathogenicity in Colletotrichum acutatum. Molecular Plant Pathology. ,vol. 7, pp. 341- 354 ,(2006) , 10.1111/J.1364-3703.2006.00341.X
Elizabeth A Winzeler, Daniel D Shoemaker, Anna Astromoff, Hong Liang, Keith Anderson, Bruno Andre, Rhonda Bangham, Rocio Benito, Jef D Boeke, Howard Bussey, Angela M Chu, Carla Connelly, Karen Davis, Fred Dietrich, Sally Whelen Dow, Mohamed El Bakkoury, Françoise Foury, Stephen H Friend, Erik Gentalen, Guri Giaever, Johannes H Hegemann, Ted Jones, Michael Laub, Hong Liao, Nicole Liebundguth, David J Lockhart, Anca Lucau-Danila, Marc Lussier, Nasiha M'Rabet, Patrice Menard, Michael Mittmann, Chai Pai, Corinne Rebischung, Jose L Revuelta, Linda Riles, Christopher J Roberts, Petra Ross-MacDonald, Bart Scherens, Michael Snyder, Sharon Sookhai-Mahadeo, Reginald K Storms, Steeve Véronneau, Marleen Voet, Guido Volckaert, Teresa R Ward, Robert Wysocki, Grace S Yen, Kexin Yu, Katja Zimmermann, Peter Philippsen, Mark Johnston, Ronald W Davis, None, Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis Science. ,vol. 285, pp. 901- 906 ,(1999) , 10.1126/SCIENCE.285.5429.901
Anna Fitzgerald, Jan A.L. van Kan, Kim M. Plummer, Simultaneous silencing of multiple genes in the apple scab fungus, Venturia inaequalis, by expression of RNA with chimeric inverted repeats. Fungal Genetics and Biology. ,vol. 41, pp. 963- 971 ,(2004) , 10.1016/J.FGB.2004.06.006
I MOUYNA, C HENRY, T DOERING, J LATGE, Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus Fems Microbiology Letters. ,vol. 237, pp. 317- 324 ,(2004) , 10.1016/J.FEMSLE.2004.06.048
Hong Liu, Tricia R Cottrell, Lynda M Pierini, William E Goldman, Tamara L Doering, None, RNA Interference in the Pathogenic Fungus Cryptococcus neoformans Genetics. ,vol. 160, pp. 463- 470 ,(2002) , 10.1093/GENETICS/160.2.463
M Orejas, E A Espeso, J Tilburn, S Sarkar, H N Arst, M A Penalva, Activation of the Aspergillus PacC transcription factor in response to alkaline ambient pH requires proteolysis of the carboxy-terminal moiety. Genes & Development. ,vol. 9, pp. 1622- 1632 ,(1995) , 10.1101/GAD.9.13.1622
B Bernstein, EI Zehr, RA Dean, E Shabi, Characteristics ofColletotrichumfrom Peach, Apple, Pecan, and Other Hosts. Plant Disease. ,vol. 79, pp. 478- 482 ,(1995) , 10.1094/PD-79-0478
J. C. Tu, An improved Mathur's medium for growth sporulation, and germination of spores of Colletotrichum lindemuthianum Microbios. ,vol. 44, pp. 87- 93 ,(1985)
Zaira Caracuel, M. Isabel G. Roncero, Eduardo A. Espeso, Clara I. González-Verdejo, Fe I. García-Maceira, Antonio Di Pietro, The pH signalling transcription factor PacC controls virulence in the plant pathogen Fusarium oxysporum Molecular Microbiology. ,vol. 48, pp. 765- 779 ,(2003) , 10.1046/J.1365-2958.2003.03465.X
C. Cogoni, J. T. Irelan, M. Schumacher, T. J. Schmidhauser, E. U. Selker, G. Macino, Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation. The EMBO Journal. ,vol. 15, pp. 3153- 3163 ,(1996) , 10.1002/J.1460-2075.1996.TB00678.X