Lack of metabolic effects of cholecystokinin on hepatocytes.

摘要: We previously reported that the liver was major organ extracts small, biologically active, circulating forms of cholecystokinin. Although our work indicated extensive degradation cholecystokinin extracted from plasma during its transit across hepatocyte, it unclear whether might also have a physiological effect on this cell before intracellular degradation. Therefore we tested hypothesis has direct biological hepatocytes. Using freshly isolated or cultured hepatocytes, studied cholecystokinin-octapeptide alters protein synthesis, affects amino acid transport influences cytosolic free calcium concentrations. slices, determined cyclic nucleotide levels. Cholecystokinin-octapeptide, up to concentration 1 mumol/L, had no incorporation radiolabeled acids into total hepatocyte protein; in contrast, comparable molar amounts insulin stimulated synthesis by as much 37% (ED50 = 1.5 x 10(-10) mol/L). and glucagon hepatocytes 14C-alpha-aminoisobutyric acid, nonmetabolizable analog, affect Cholecystokinin-octapeptide did not either individual measured digitized video microscopy using Fura-2, levels AMP GMP slices. Our results show These previous data suggest primary outcome hepatic extraction is hormone