Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold
作者： Masakazu Yamada , Takahisa Anada , Taisuke Masuda , Teruko Takano-Yamamoto , Osamu Suzuki
DOI: 10.1016/J.SNB.2015.05.073
关键词:
摘要: Abstract We report the extent of mechanical stress on a bioactive three-dimensional scaffold that can induce differentiation seeded osteoblastic cells in vitro. Mouse mesenchymal stem D1 were porous spongy consists octacalcium phosphate (OCP) and gelatin (Gel) composite, which was previously proven to be sufficient bone-replaceable material vivo. The cellular activity determined after loading uni-axial compression by deforming 5 mm ring-shaped, cell-seeded OCP/Gel composite range 1–3 mm (corresponding 20–60% thickness) using cyclic device designed for this study. deformation did not affect proliferation. However, enhancement marker genes, such as osteopontin osteoclacin, occurred deformed just 20%. These results suggest is promoted if suitable provided, indicates biological relevance bone regenerative properties under shown previous vivo
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sci-hub.se 参考文章(45)
Friedrich Pauwels, A New Theory Concerning the Influence of Mechanical Stimuli on the Differentiation of the Supporting Tissues Biomechanics of Locomotor Apparatus. pp. 375- 407 ,(1980) , 10.1007/978-3-642-67138-8_14
Takahisa Anada, Takashi Kumagai, Yoshitomo Honda, Taisuke Masuda, Ryutaro Kamijo, Shinji Kamakura, Nobuhito Yoshihara, Tsunemoto Kuriyagawa, Hidetoshi Shimauchi, Osamu Suzuki, Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells. Tissue Engineering Part A. ,vol. 14, pp. 965- 978 ,(2008) , 10.1089/TEN.TEA.2007.0339
Angel O. Duty, Megan E. Oest, Robert E. Guldberg, Cyclic mechanical compression increases mineralization of cell-seeded polymer scaffolds in vivo Journal of Biomechanical Engineering-transactions of The Asme. ,vol. 129, pp. 531- 539 ,(2007) , 10.1115/1.2746375
Masamichi Takami, Ayako Mochizuki, Atsushi Yamada, Keita Tachi, Baohong Zhao, Yoichi Miyamoto, Takahisa Anada, Yoshitomo Honda, Tomio Inoue, Masanori Nakamura, Osamu Suzuki, Ryutaro Kamijo, Osteoclast Differentiation Induced by Synthetic Octacalcium Phosphate Through Receptor Activator of NF-κB Ligand Expression in Osteoblasts Tissue Engineering Part A. ,vol. 15, pp. 3991- 4000 ,(2009) , 10.1089/TEN.TEA.2009.0065
H. Imaizumi, M. Sakurai, O. Kashimoto, T. Kikawa, O. Suzuki, Comparative Study on Osteoconductivity by Synthetic Octacalcium Phosphate and Sintered Hydroxyapatite in Rabbit Bone Marrow Calcified Tissue International. ,vol. 78, pp. 45- 54 ,(2006) , 10.1007/S00223-005-0170-0
Efstathios Michalopoulos, Richard L. Knight, Sotirios Korossis, John N. Kearney, John Fisher, Eileen Ingham, Development of methods for studying the differentiation of human mesenchymal stem cells under cyclic compressive strain. Tissue Engineering Part C-methods. ,vol. 18, pp. 252- 262 ,(2012) , 10.1089/TEN.TEC.2011.0347
Michael J. Gardner, Marjolein C. H. van der Meulen, Demetris Demetrakopoulos, Timothy M. Wright, Elizabeth R. Myers, Mathias P. Bostrom, In vivo cyclic axial compression affects bone healing in the mouse tibia Journal of Orthopaedic Research. ,vol. 24, pp. 1679- 1686 ,(2006) , 10.1002/JOR.20230
R. Sapir-Koren, G. Livshits, Osteocyte control of bone remodeling: is sclerostin a key molecular coordinator of the balanced bone resorption-formation cycles? Osteoporosis International. ,vol. 25, pp. 2685- 2700 ,(2014) , 10.1007/S00198-014-2808-0
S. Kamakura, Y. Sasano, H. Homma-Ohki, M. Nakamura, O. Suzuki, M. Kagayama, K. Motegi, Multinucleated giant cells recruited by implantation of octacalcium phosphate (OCP) in rat bone marrow share ultrastructural characteristics with osteoclasts Journal of Electron Microscopy. ,vol. 46, pp. 397- 403 ,(1997) , 10.1093/OXFORDJOURNALS.JMICRO.A023535
WALTER E. BROWN, JAMES P. SMITH, JAMES R. LEHR, A. WILLIAM FRAZIER, Octacalcium Phosphate and Hydroxyapatite: Crystallographic and Chemical Relations between Octacalcium Phosphate and Hydroxyapatite Nature. ,vol. 196, pp. 1050- 1055 ,(1962) , 10.1038/1961050A0