Protective effects of All-trans-retinoic acid against cardiac arrhythmias induced by isoproterenol, lysophosphatidylcholine or ischemia and reperfusion.

摘要: Previous studies have shown that free polyunsaturated fatty acids (PUFA) reduce the excitability of cardiac myocytes and exert antiarrhythmic effects. Therefore, we hypothesized retinoic acid (RA, vitamin A acid), which has structural characteristics similar to those PUFA, may To test this hypothesis, used an isolated, spontaneously beating, neonatal rat myocyte preparation examine effects RA, added perfusion solution, on cell contraction arrhythmias induced by isoproterenol (ISO) or lysophosphatidylcholine (LPC). All-trans-RA (10-20 microM) a marked reversible reduction in rate 2-5 min without changing amplitude contractions. Superfusion with either ISO (3 LPC (5 sustained tachyarrhythmias characterized spasmodic contractures fibrillation. Addition 15-20 microM all-trans-RA solution effectively prevented as well terminated LPC. Furthermore, whole-animal model arrhythmia left anterior descending coronary artery (LAD) anesthetized was occluded for 15 followed reperfusion, both incidence severity ventricular tachycardia fibrillation (VT, VF) were significantly reduced during ischemic reperfusion periods intravenous infusion all-trans-RA. In contrast, other analogues, including retinol retinal, fat-soluble vitamins, D, E, K, did not such Our results demonstrate can produce suggesting novel role RA potential agent.