作者: Bruce C. Kirkpatrick , Christine D. Smart
DOI: 10.1016/S0065-2296(08)60013-0
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摘要: Publisher Summary During the past decades, application of recombinant DNA technologies has greatly expanded knowledge about genetic diversity plant pathogenic mycoplasma-like organisms (MLOs). Since discovery that phloem-limited, wall-less prokaryotes, and not viruses, were causal agents numerous graft transmissible “yellows diseases”, much effort been expended trying to culture these pathogens in vitro . With exception Thermoplasma, most prokaryotes are members Class Mollicutes. Sequence analysis two other evolutionary markers confirms conclusions obtained by 16s rRNA phylogenetic analyses. The inability phytoplasmas impeded experimental manipulation pathogens. Given their small genome size lack a cell wall, Mollicutes might seem be ideal candidates for molecular number variety methods introduce manipulate genes interest progressed recent years. Significant progress made understanding determinants pathogenicity hosts some culturable, Gram-negative phytopathogenic prokaryotes. Phylogenetic analyses aster yellows (AY) maize bushy stunt (MBS) have shown very closely related. Transformed WX could injected into C. montunus which would fed on peach seedlings.