Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography

作者: Jinxin Fan , Jianquan Luo , Yinhua Wan

DOI: 10.1016/J.CEJ.2017.06.172

关键词:

摘要: Abstract Biocatalytic membranes are promising to remove micro-pollutants in aqueous environment due their mild and green operation condition. However, more efforts need be devoted improving removal efficiency stability. In this study, metal chelating affinity membrane chromatography (MCAMC) was used construct a biocatalytic by selectively capturing laccase from crude fermentation broth. Metal ions had significant effect on the activity of immobilized copper ion best choice. A pH 4.5 selected for adsorption its loading seemed same under flow rates 0.5 10 mL min −1 thanks inherent convective transport chromatography. The value salt concentration storage buffer an obvious stability laccase, prepared retained 87% initial after 20 days storage. When applying such micro-pollutant (taking bisphenol (BPA) as example), high BPA (99.3%) could obtained. operated at flux 50 L m −2  h without recycling permeate into feed, throughput rate were superior most results literature. decline (from 99.6% 56.6% five cycles) occurred during successive water treatment fouling caused polymerization products. Membrane regeneration achieved simple elution-cleaning-reloading, fully recovered.

参考文章(39)
Koichi Sawada, Shinji Sakai, Masahito Taya, Electrochemical recycling of gold nanofibrous membrane as an enzyme immobilizing carrier Chemical Engineering Journal. ,vol. 280, pp. 558- 563 ,(2015) , 10.1016/J.CEJ.2015.06.029
Fei Liu, Qian Liu, Yanhong Zhang, Yanjie Liu, Yingchun Wan, Kaichun Gao, Ying Huang, Wei Xia, Haiying Wang, Yun Shi, Zheng Huang, Bin Lu, Molecularly imprinted nanofiber membranes enhanced biodegradation of trace bisphenol A by Pseudomonas aeruginosa Chemical Engineering Journal. ,vol. 262, pp. 989- 998 ,(2015) , 10.1016/J.CEJ.2014.10.046
Valerie Orr, Luyang Zhong, Murray Moo-Young, C. Perry Chou, Recent advances in bioprocessing application of membrane chromatography Biotechnology Advances. ,vol. 31, pp. 450- 465 ,(2013) , 10.1016/J.BIOTECHADV.2013.01.007
Raja Ghosh, Protein separation using membrane chromatography: opportunities and challenges Journal of Chromatography A. ,vol. 952, pp. 13- 27 ,(2002) , 10.1016/S0021-9673(02)00057-2
Chih-I Chen, Yi-Miao Ko, Wei-Ling Lien, Yi-Hsuan Lin, I-Tsang Li, Chien-Hsun Chen, Chwen-Jen Shieh, Yung-Chuan Liu, Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane Journal of Membrane Science. ,vol. 401, pp. 33- 39 ,(2012) , 10.1016/J.MEMSCI.2012.01.022
Tingting Xia, Yueping Guan, Mingzhu Yang, Wubin Xiong, Ning Wang, Shen Zhao, Chen Guo, Synthesis of polyethylenimine modified Fe3O4 nanoparticles with immobilized Cu2+ for highly efficient proteins adsorption Colloids and Surfaces A: Physicochemical and Engineering Aspects. ,vol. 443, pp. 552- 559 ,(2014) , 10.1016/J.COLSURFA.2013.12.026
Müfrettin Sarı, Sinan Akgöl, Melike Karataş, Adil Denizli, Reversible Immobilization of Catalase by Metal Chelate Affinity Interaction on Magnetic Beads Industrial & Engineering Chemistry Research. ,vol. 45, pp. 3036- 3043 ,(2006) , 10.1021/IE0507979
Qingqing Wang, Jing Cui, Guohui Li, Jinning Zhang, Fenglin Huang, Qufu Wei, Laccase Immobilization by Chelated Metal Ion Coordination Chemistry Polymers. ,vol. 6, pp. 2357- 2370 ,(2014) , 10.3390/POLYM6092357
JERKER PORATH, JAN CARLSSON, INGMAR OLSSON, GRETA BELFRAGE, Metal chelate affinity chromatography, a new approach to protein fractionation Nature. ,vol. 258, pp. 598- 599 ,(1975) , 10.1038/258598A0