作者: Jinxin Fan , Jianquan Luo , Yinhua Wan
DOI: 10.1016/J.CEJ.2017.06.172
关键词:
摘要: Abstract Biocatalytic membranes are promising to remove micro-pollutants in aqueous environment due their mild and green operation condition. However, more efforts need be devoted improving removal efficiency stability. In this study, metal chelating affinity membrane chromatography (MCAMC) was used construct a biocatalytic by selectively capturing laccase from crude fermentation broth. Metal ions had significant effect on the activity of immobilized copper ion best choice. A pH 4.5 selected for adsorption its loading seemed same under flow rates 0.5 10 mL min −1 thanks inherent convective transport chromatography. The value salt concentration storage buffer an obvious stability laccase, prepared retained 87% initial after 20 days storage. When applying such micro-pollutant (taking bisphenol (BPA) as example), high BPA (99.3%) could obtained. operated at flux 50 L m −2 h without recycling permeate into feed, throughput rate were superior most results literature. decline (from 99.6% 56.6% five cycles) occurred during successive water treatment fouling caused polymerization products. Membrane regeneration achieved simple elution-cleaning-reloading, fully recovered.