Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane
作者： Chih-I Chen , Yi-Miao Ko , Wei-Ling Lien , Yi-Hsuan Lin , I-Tsang Li
DOI: 10.1016/J.MEMSCI.2012.01.022
关键词: Long period 、 Membrane 、 Chromatography 、 Residual activity 、 Metal 、 Phosphate buffered saline 、 Penicillin G Acylase 、 Immobilized enzyme 、 Chemistry 、 Stripping (chemistry)
摘要: Abstract In this study, an immobilized metal ion affinity membrane (IMAM) was prepared and used to immobilize penicillin G acylase (PGA). The stability of storage the PGA (IPM) tested. When stored in deionized water (DI) at 4 °C, only 19% residual activity IPM retained for 10 days. However, when 10 mM phosphate buffer (PB, pH 8) with 0.1% NaN 3 , can retain 99% its a 10-day reaction test. feasibility regeneration after long period investigated. using 100 mM EDTA as stripping solution, re-immobilized capacity is 52% original one 4 regenerations. process modified by solution (300 mM NaCl, 25 mM EDTA, 20 mM PB, 30 min, then immersed 0.5 M HCl NaOH respectively 10 min, resulting IMAM reserve 5
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sci-hub.se 参考文章(25)
Yongjun Lü, Yanglong Guo, Yanqin Wang, Xiaohui Liu, Yunsong Wang, Yun Guo, Zhigang Zhang, Guanzhong Lu, Immobilized penicillin G acylase on mesoporous silica: The influence of pore size, pore volume and mesophases Microporous and Mesoporous Materials. ,vol. 114, pp. 507- 510 ,(2008) , 10.1016/J.MICROMESO.2007.12.027
Yung-Chuan Liu, Chih-Chiang ChangChien, Shing-Yi Suen, Purification of penicillin G acylase using immobilized metal affinity membranes Journal of Chromatography B. ,vol. 794, pp. 67- 76 ,(2003) , 10.1016/S1570-0232(03)00417-3
Cesar Mateo, Gloria Fernández-Lorente, Olga Abian, Roberto Fernández-Lafuente, Jose M. Guisán, Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules. ,vol. 1, pp. 739- 745 ,(2000) , 10.1021/BM000071Q
Dariush Norouzian, Sedigheh Javadpour, Nasrin Moazami, Azim Akbarzadeh, Immobilization of whole cell penicillin G acylase in open pore gelatin matrix Enzyme and Microbial Technology. ,vol. 30, pp. 26- 29 ,(2002) , 10.1016/S0141-0229(01)00445-8
Luciana Cristina Lins de Aquino, Heloisa Ribeiro Tunes de Sousa, Everson Alves Miranda, Luciano Vilela, Sônia Maria Alves Bueno, Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin. Journal of Chromatography B. ,vol. 834, pp. 68- 76 ,(2006) , 10.1016/J.JCHROMB.2006.02.015
Rodrigo Torres, Benevides Pessela, Manuel Fuentes, Roberto Munilla, Cesar Mateo, Roberto Fernández-Lafuente, José M Guisán, None, Stabilization of enzymes by multipoint attachment via reversible immobilization on phenylboronic activated supports Journal of Biotechnology. ,vol. 120, pp. 396- 401 ,(2005) , 10.1016/J.JBIOTEC.2005.06.017
D. Bianchi, P. Golini, R. Bortolo, P. Cesti, Immobilization of penicillin G acylase on aminoalkylated polyacrylic supports Enzyme and Microbial Technology. ,vol. 18, pp. 592- 596 ,(1996) , 10.1016/0141-0229(95)00149-2
Jing He, Xiaofen Li, D.G Evans, Xue Duan, Chengyue Li, A new support for the immobilization of penicillin acylase Journal of Molecular Catalysis B: Enzymatic. ,vol. 11, pp. 45- 53 ,(2000) , 10.1016/S1381-1177(00)00218-6
Ping Xue, Guanzhong Lu, Yanglong Guo, Yunsong Wang, Yun Guo, A novel support of MCM-48 molecular sieve for immobilization of penicillin G acylase Journal of Molecular Catalysis B-enzymatic. ,vol. 30, pp. 75- 81 ,(2004) , 10.1016/J.MOLCATB.2004.03.010
Y LIU, S SUEN, C HUANG, C CHANGCHIEN, Effects of spacer arm on penicillin G acylase purification using immobilized metal affinity membranes Journal of Membrane Science. ,vol. 251, pp. 201- 207 ,(2005) , 10.1016/J.MEMSCI.2004.11.018