Affinity electromembrane with covalently coupled heparin for thrombin adsorption
作者： Jun Wang , Guisen Wu , Wei Shi , Xin Liu , Changqing Ruan
DOI: 10.1016/J.MEMSCI.2012.11.015
关键词:
摘要: Abstract A new composite membrane with electrical conductivity and reactive groups (–COOH) was synthesized by copolymerizing pyrrole-3-carboxylic acid pyrrole onto nylon via the template method. The ligand, heparin, covalently coupled activating N-hydroxysuccinimide 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide modified ethylenediamine. as-synthesized affinity electromembrane exhibited a uniform value of 3.7×10 −7 S/cm content heparin approximately 1.897 mg/g membrane. By applying an stimulation (−0.5 V) to electromembrane, adsorption capacity rate were greatly enhanced. Elution protein showed desorption ratio up 92.7% using 2.0 M NaSCN solution as agent. To investigate performance dynamic heparin-coupled cartridge which can be applied designed. result separation that time breakthrough delayed from 22 min in absence 31 min presence stimulation.
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