Hormonal and ionic control of the glycogenolytic cascade in rat liver

摘要: 1. A parallel dose-dependent activation of histone kinase, phosphorylase kinase and was observed in isolated hepatocytes incubated the presence glucagon; effect suboptimal concentrations glucagon antagonized by insulin. 2. An which not accompanied a stable change activity with phenylephrine, isoproterenol or vasopressin as well on decapitation unanesthetized animals. dissociation two enzymic activities also high concentration glucose, strongly inactivated no kinase. 3. The phenylephrine counteracted insulin, greatly decreased absence Ca2+ from incubation medium, completely suppressed replacement Na+ K+. 4. In liver extract, could be activated trypsin. Control, glucagon-activated trypsin-activated inhibited about 70% EGTA restored addition Ca2+. 5. mechanisms that control are discussed.