Impact of saliva collection methods on sIgA and cortisol assays and acceptability to participants

作者: Lyndall Strazdins , Shannon Meyerkort , Vicki Brent , Rennie M. D'Souza , Dorothy H. Broom

DOI: 10.1016/J.JIM.2005.09.010

关键词:

摘要: In community-based studies of stress and immunity, saliva samples offer a non-intrusive way gathering biological data. Cotton-based devices are widely used in cortisol research, but some may affect assay results. We compared reliability perceived acceptability three collection methods: passive, cotton 'salivettes' cellulose-cotton tip 'eyespears'. Compared to passive collection, salivettes reduced the concentration (p = .001) sIgA .002). Eyespears did not reduce or concentration, showed less interference rank ordering (r(eyespear with passive) .90) scores .96) (r cortisol(salivette .79; r sIgA(salivette .66). The comfort both cotton-based were rated positively. Cotton-cellulose eyespears could methodological advantages for collecting measure levels, and, because they can be held during sampling, useful research children frail elderly.

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