Dynamic Protein Interactions of the Polycomb Repressive Complex 2 during Differentiation of Pluripotent Cells

摘要: Polycomb proteins assemble to form complexes with important roles in epigenetic regulation. The Repressive Complex 2 (PRC2) modulates the di- and tri-methylation of lysine 27 on histone H3, each which are associated gene repression. Although three subunits, EZH1/2, SUZ12, EED, catalytic core PRC2, a wider group associate low stoichiometry. This raises question whether dynamic variation PRC2 interactome results alternative forms complex during differentiation. Here we compared physical interactions undifferentiated differentiated states NTERA2 pluripotent embryonic carcinoma cells. Label-free quantitative proteomics was used assess endogenous immunoprecipitation EZH2 SUZ12 subunits PRC2. A high stringency data set reflecting state produced that included all previously reported components, several novel interacting proteins. Comparison interactomes obtained cells revealed candidate were enriched isolated from one two states. For example, SALL4 ZNF281 cells, whereas PCL1 SMAD3 preferentially differentiating Analysis mRNA protein levels these factors their association correlated cell state-specific expression. Taken together, propose changes differentiation may contribute directing its activity fate transitions.