Transcriptome sequencing of three Ranunculus species (Ranunculaceae) reveals candidate genes in adaptation from terrestrial to aquatic habitats.

摘要: Adaptation to aquatic habitats is a formidable challenge for terrestrial angiosperms that has long intrigued scientists. As part of suite work explore the molecular mechanism adaptation habitats, we here sequenced transcriptome submerged plant Ranunculus bungei, and two relatives R. cantoniensis brotherusii, followed by comparative evolutionary analyses determine candidate genes adaption habitats. We obtained 126,037, 140,218 114,753 contigs brotherusii respectively. Bidirectional Best Hit method OrthoMCL identified 11,362 8,174 1:1:1 orthologous (one ortholog represented in each three species) Non-synonymous/synonymous (dN/dS) were performed with maximum likelihood an approximate species-pairs. In total, 14 bungei potentially involved adaptive transition from identified. Some homologs these model plants are vacuole protein formation, regulating 'water transport process' 'microtubule cytoskeleton organization'. Our study opens door understand