Sequential assignment of the backbone nuclei (1H,15N and13C) of c-H-ras p21 (1–166).GDP using a novel 4D NMR strategy
作者： Sharon L. Campbell-Burka , Peter J. Domaillea , Melissa A. Starovasnik , Wayne Boucher , Ernest D. Laue
DOI: 10.1007/BF02192852
关键词:
摘要: The c-H-ras p21 protein is the product of humanras proto-oncogene, a member ubiquitous eukaryotic gene family which highly conserved in evolution. These proteins play an important role control cellular growth. We report here sequential assignment backbone nuclei truncated form 21-kD product, using our recently proposed 4D NMR strategy (Boucher et al., 1992). assignments are first step towards full investigation structure, dynamics and interactions wild-type oncogenicrasp21 spectroscopy. Some data were presented at 33rd ENC held Asilomar, California, U.S.A., April 1992.
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sci-hub.se 参考文章(35)
Sharon Campbell-Burk, Structural and dynamic differences between normal and transforming N-ras gene products: a 31P and isotope-edited 1H NMR study. Biochemistry. ,vol. 28, pp. 9478- 9484 ,(1989) , 10.1021/BI00450A035
J John, I Schlichting, E Schiltz, P Rösch, A Wittinghofer, C-terminal truncation of p21H preserves crucial kinetic and structural properties Journal of Biological Chemistry. ,vol. 264, pp. 13086- 13092 ,(1989) , 10.1016/S0021-9258(18)51599-4
Roymarie Ballester, Douglas Marchuk, Mark Boguski, Ann Saulino, Roxanne Letcher, Michael Wigler, Francis Collins, The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. Cell. ,vol. 63, pp. 851- 859 ,(1990) , 10.1016/0092-8674(90)90151-4
Wayne Boucher, Ernest D. Laue, Sharon Campbell-Burk, Peter J. Domaille, Four-dimensional heteronuclear triple resonance NMR methods for the assignment of backbone nuclei in proteins Journal of the American Chemical Society. ,vol. 114, pp. 2262- 2264 ,(1992) , 10.1021/JA00032A053
E.D Laue, M.R Mayger, J Skilling, J Staunton, Reconstruction of phase-sensitive two-dimensional NMR spectra by maximum entropy Journal of Magnetic Resonance. ,vol. 68, pp. 14- 29 ,(1986) , 10.1016/0022-2364(86)90312-4
A. T. Brunger, M. V. Milburn, L. Tong, A. M. deVos, J. Jancarik, Z. Yamaizumi, S. Nishimura, E. Ohtsuka, S. H. Kim, Crystal structure of an active form of RAS protein, a complex of a GTP analog and the HRAS p21 catalytic domain. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 4849- 4853 ,(1990) , 10.1073/PNAS.87.12.4849
Ilme Schlichting, Jacob John, Mathias Frech, Pierre Chardin, Alfred Wittinghofer, Herbert Zimmermann, Paul Roesch, Proton NMR studies of transforming and nontransforming H-ras p21 mutants. Biochemistry. ,vol. 29, pp. 504- 511 ,(1990) , 10.1021/BI00454A026
Dominique Marion, Mitsuhiko Ikura, Ad Bax, Improved solvent suppression in one-and two-dimensional NMR spectra by convolution of time-domain data Journal of Magnetic Resonance. ,vol. 84, pp. 425- 430 ,(1989) , 10.1016/0022-2364(89)90391-0
Ad Bax, G.Marius Clore, Angela M Gronenborn, 1H1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins Journal of Magnetic Resonance. ,vol. 88, pp. 425- 431 ,(1990) , 10.1016/0022-2364(90)90202-K
Alfred G. Redfield, Mary Z. Papastavros, NMR study of the phosphoryl binding loop in purine nucleotide proteins: evidence for strong hydrogen bonding in human N-ras p21. Biochemistry. ,vol. 29, pp. 3509- 3514 ,(1990) , 10.1021/BI00466A013