Rapid visualization of hydrogen positions in protein neutron crystallographic structures
作者： Parthapratim Munshi , Shang-Lin Chung , Matthew P. Blakeley , Kevin L. Weiss , Dean A. A. Myles
DOI: 10.1107/S0907444911048402
关键词:
摘要: Neutron crystallography is a powerful technique for experimental visualization of the positions light atoms, including hydrogen and its isotope deuterium. In recent years, structural biologists have shown increasing interest in as it uniquely complements X-ray crystallographic data by revealing D atoms macromolecules. With this regained interest, access to macromolecular neutron beamlines becoming limiting step. report, it that rapid data-collection strategy can be valuable alternative longer times appropriate cases. Comparison perdeuterated rubredoxin structures refined against sets collected over hours up 5 d shows collection just 14 h sufficient provide 269 without ambiguity.
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