Cloning, genetic characterization, and chromosomal mapping of the mouse VDUP1 gene.

摘要: Abstract VDUP1 encodes a vitamin D3-inducible gene product that has been shown to be down-regulated in chemically-induced mammary tumors rats. It recently reported negatively regulate thioredoxin expression and function. We have cloned the mouse characterized its genomic locus. The coding region spans eight exons within total length of 2.3 kb located on chromosome 3. Consensus sites for polyadenylation were identified 1.3 downstream gene, defining long 3′ untranslated region. minimal functional promoter contains TATA CCAAT boxes transcription is initiated from two major start downstream. A direct repeat element proximal with homology USF binding site was as potential regulator VDUP1gene expression. Expression analysis determined mRNA markedly induced myeloma cells high density cell culture, but not sub-confluent arrested by serum deprivation. All samples panel immortalized or transformed lines express abundant levels mRNA.