Polar Body Genome Transfer for Preventing the Transmission of Inherited Mitochondrial Diseases

摘要: Inherited mtDNA diseases transmit maternally and cause severe phenotypes. Currently, there is no effective therapy or genetic screens for these diseases; however, nuclear genome transfer between patients' healthy eggs to replace mutant mtDNAs holds promises. Considering that a polar body contains few mitochondria shares the same genomic material as an oocyte, we perform prevent transmission of variants. We compare effects different types germline transfer, including spindle-chromosome pronuclear first second in mice. Reconstructed embryos support normal fertilization produce live offspring. Importantly, analysis confirms F1 generation from possesses minimal donor carryover compared other procedures. Moreover, genotype remains stable F2 progeny after transfer. Our preclinical model demonstrates has great potential inherited diseases.