Some properties of a hitherto undescribed filamentous virus of the grapevine

作者: M. A. Castellano , D. Boscia , E. Choueiri , G. P. Martelli , M. Digiaro

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摘要: An apparently new, non mechanically-transmissible clostero-like virus, for which the name ''grapevine leafroll-associated virus 7('') (GLRaV-7) is proposed, was found in Albanian grapevine accessions. Virus particles were filamentous, had conspicuous cross banding and a length of 1500-1700 nm. Virions coat protein subunits with an estimated M(r) ca. 37 kDa ssRNA genome size 19.5 kb as deduced from estimate dsRNA (ca. kbp) extracted tissues. A virus-specific antiserum raised, decorated virions at dilution 1:1000. This did not recognize any six viruses (GLRaV-1 to -6) known date, nor trichovirus (GVA) B (GVB). Grapevine indicators graft-inoculated material accessions containing GLRaV-7 reacted mild leafroll-like symptoms. In survey 2226 vines 30 different countries examined by ELISA, 141 plants Albania, Greece, Hungary, Egypt, Italy.

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