Instrumental Assay of Microbial Lipase at Constant pH1

摘要: A rapid, accurate method with high sensitivity and reproducibility, having the advantage of a short incubation period under constant pH, has been developed for routine measurement microbial lipase. Assembled from readily available economical instrumental components, apparatus includes pH meter, thermoelectric heating stirring device, motor-driven burette, an automatic recorder. The reaction mixture, consisting 5 ml 10% olive oil-gum arabic emulsion, 2 3 m NaCl, sodium taurocholate (15 mg/ml) 0.075 CaCl2, water, 1 enzyme solution, was adjusted to 8.0 37 C. maintained at value by addition 0.01 n NaOH during period, which usually lasted min. lipase unit, derived use this technique, may be defined as number microequivalents acid liberated per minute specified conditions. sensitive units. Various organisms tested produced 0.17 1.32 units cell filtrate. An Arrhenius plot staphylococcal yielded 14,500 cal function (energy activation).