Growth hormone (GH) is a survival rather than a proliferative factor for embryonic striatal neural precursor cells.

摘要: Abstract Objective A possible role of GH during central nervous system (CNS) development has been suggested by the presence this hormone and its receptor in brain areas before production pituitary gland. Although several effects have reported for GH, specific CNS remains unclear. Here, we examined effect on proliferation, survival neurosphere formation primary cultures striatal tissue from14-day-old (E14) mouse embryos. Design gene expression was confirmed RT-PCR. Primary embryonic cells were treated with different doses serum free media, then number neurospheres determined. To examine proliferation cultures, bromodeoxyuridine (BrdU) TUNEL immunoreactivity conducted. Results In epidermal growth factor (EGF), increased neurospheres, a maximal response at 10 ng/ml, higher inhibitory. absence EGF, failed to stimulate formation. Proliferation rate inhibited 24 or 48 h incubation GH. However, BrdU incorporation. treatment decreases apoptosis nestin GFAP positive enhancing Conclusions Our vitro data demonstrate that plays original population cells, improving Neural Precursor Cells (NPCs) expansion. We suggest action could be predominant neurodevelopment.