Real-time assessment of hybridization between wolves and dogs: combining noninvasive samples with ancestry informative markers.

摘要: Wolves and dogs provide a paradigmatic example of the ecological conservation implications hybridization events between wild domesticated forms. However, our understanding such has been traditionally hampered by both high genetic similarity difficulties in obtaining tissue samples (TS), which limit ability to assess ongoing events. To occurrence extension pack wolf-dog hybrids northwestern Iberia, we compared power 52 nuclear markers implemented on TS with subset 13 ancestry informative (AIMs) typed noninvasive (NIS). We demonstrate that AIMs are as accurate were chosen without regard differentiate wolves dogs, also having advantage being rapidly screened NIS. The efficiency significantly outperformed ten random sets similar size an additional commercial set 18 markers. Bayesian clustering analysis NIS identified nine hybrids, two dogs. Four unambiguously assigned F1xWolf backcrosses. Our approach (AIMs + NIS) overcomes previous related sample availability markers, allowing quick identification first phases episodes. This provides managers reliable tool evaluate estimate success their actions. may be easily adapted for other pairs wild/domesticated species, thus improving introgression domestication genes into natural populations.