Method for determining a degree of mastitis infection

摘要: A method for determining a degree of mastitis infection comprising the steps i) preparing sample uninsulated mammalian milk in somatic cells are not isolated particles interference by adding reagent differentiation marker staining meta-chromatic, an amount sufficient to provide distinction between types at dilution ratio less than 1 quantity: 200; ii) measuring cell count each one or more leukocytes selected group type lymphocytes, monocytes, macrophages and polymorphonuclear leukocyte differentiated through flow cytometer having system sensitive differences resulting from differential association with different detection; iii) determine indication based on percentage total number measures as measured comparing reference values, predetermined be associated degrees infection.