Dual protection of hydroxytyrosol, an olive oil polyphenol, against oxidative damage in PC12 cells

摘要: Hydroxytyrosol (3,4-dihydroxyphenylethanol, HT), a major polyphenol in olive oils, has received increasing attention due to its multiple pharmacological activities. However, it is not well understood how HT works on the neuronal system. We report herein that efficiently scavenges free radicals vitro and displays cytoprotection against oxidative stress-induced damage PC12 cells. completely protects cells from hydrogen peroxide-induced death rescues 6-hydroxydopamine-induced damage. Mechanistic studies reveal Nrf2 prerequisite for neuroprotection of as knocking down eliminated this action. HT, via activation Keap1-Nrf2 pathway, elevates panel cytoprotective enzymes, including glutamate–cysteine ligase, HO-1, NQO1 thioredoxin reductase. Our study reveals provides dual cellular antioxidant defense both radical scavenger activator, suggesting potential pharmaceutical usage treatment neurodegenerative disorders.