Mechanism of photoinduced carotenoid synthesis. Induction of carotenoid synthesis by antimycin A in the absence of light in mycobacterium marinum.

摘要: Abstract Mycobacterium marinum, like a number of nonphotosynthetic microorganisms, produces only traces carotenoids when grown in the dark. On illumination, dark bacteria synthesize substantial amounts de novo. This study shows that antimycin A can also induce synthesis M. marinum absence light. The synthesized are proportional to amount added; for maximal carotenogenesis, concentration 37.5 µm is required. Antimycin 300 400% more effective than saturating light induction carotenoid synthesis. effects and additive. Chromatography pigments reveals same polyenes regardless means induction—light alone, dark, or plus Evidence presented both act by inducing protein (carotenogenic enzymes). In A-induced system continues 20 hours. light-induced system, on other hand, 4 hours then stops. However, second illumination at this time lead resumption Inasmuch as inductive additive, it concluded sites action different. may either an inducer carotenogenic enzymes inactivating repressor. Light have analogous role but different site; photo-oxidizing repressor producing photo-oxidized metabolite which enzymes.