[Light-dependent carotenoid synthesis : II. Substitution of photoinduction by mercuribenzoate].

摘要: In the light-dependent carotenoid synthesis in Fusarium aquaeductuum photoinduction can be substituted by incubation of mycelium with SH-group poisons p-chloro-and p-hydroxymercuribenzoate dark. The minimum concentration both substances effective for induction is 5.10(-6) molar; maximal production pigments occurs at molar 5.10(-5)-10(-4). incubated mercuribenzoate, starts after a lagperiod 4-6 hours, increases until 24 hours addition poison and then continues some days constant rate. result that lag-period treatment mercuribenzoate much longer than it may due to fact presence respiration severely decreased (to 25% normal value concentrations 5.10(-5)) as consequence other metabolic processes are also depressed. From experiments which p-hydroxy-mercuribenzoate was removed different times washing distilled water, seen even an time only 2 induction. Other blocking do not induce synthesis; mercuring compounds benzoic acid ineffective.Carotenoid induced illumination or mercuri-benzoate quantitatively inhibited actidion (cycloheximid), potent inhibitor protein fungi. inhibition decreases increasing between beginning actidion; from this concluded reaction carotenogenic enzymes newly formed. If added 3 there almost no pigment production, indicating itself actidion.From these results light catalyse same chain regulatory mechanism, namely, SH-groups. We therefore assume function mechanism photooxydize SH-groups specific compound. Several possibilities compound site discussed.