The chemokine receptor CCR4 is expressed and associated with a poor prognosis in patients with gastric cancer.

摘要: Objectives: There is increasing evidence that chemokines and chemokine receptors are causally involved in the metastasis of cancer. Little known about possible role gastric The aim this study was to investigate expression their prognostic patients with Methods: We screened CCR CXCR 12 cancer cell lines using semi-quantitative RT-PCR. CCR4, one most commonly expressed chemokines, confirmed Western blot flow cytometry analysis 8 lines. function CCR4 examined migration proliferation assays. Then migratory response blocked blocking antibodies. Finally, clinical significance explored tissue microarray methods immunohistochemical staining specimens from 753 patients. Results: found 6 out (75.0%) carcinoma a functional for its ligand, CCL17, as demonstrated by assays, inhibited anti-CCR4 samples evaluated assay microarrays showed CCR4-positive cells were detected 128 (17.0%) cases. In addition, there significant difference recurrences between -negative cases (P = 0.009). tumors had significantly poorer prognosis than did those CCR4-negative (5-year survival rate; 71.6% versus 82.5%, respectively, P 0.008). Conclusion: These results suggest ligands associated increased tumor recurrence impaired overall