Characterization and expression patterns of ERK1 and ERK2 from Epinephelus coioides against Cryptocaryon irritans infection.
作者： Hong-Yan Sun , Mian-Zhi Huang , Ze-Quan Mo , Liang-Shi Chen , Guo Chen
DOI: 10.1016/J.FSI.2017.12.050
关键词:
摘要: Abstract Mitogen-activated protein kinases (MAPKs), a group of serine-threonine kinases, play crucial role in immunoreaction response to extra environmental stresses. In this study, two novel MAPKs, Ec-ERK1 and Ec-ERK2, were identified from Epinephelus coioides. Both Ec-ERK2 sequences contain highly conserved Thr-Glu-Tyr (TEY) motif, an HRD domain, ATP binding loop containing GXGXXG. An analysis phylogenetic relationships demonstrated that ERK amino acid between different species indicating the functions may be similar. mRNA can detected all thirteen tissues examined, but expression level is these tissues. The patterns genes E. coioides also against Cryptocaryon irritans infection, which capable killing large numbers fish short time has serious impact on aquaculture. was up-regulated most with highest expressions (3.9 times) occurring head kidney (3.5 spleen. There no significant correlation Ec-ERK1/Ec-ERK2 nuclear factor kappaB (NF-kB). results indicated characters Ec-ERK1/ERK2 conserved, showed tissue-specific healthy grouper, their significantly varied post C. suggesting important roles during pathogen-caused inflammation.
sci-hub.se 参考文章(46)
D. M. Payne, A. J. Rossomando, P. Martino, A. K. Erickson, J. H. Her, J. Shabanowitz, D. F. Hunt, M. J. Weber, T. W. Sturgill, Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase). The EMBO Journal. ,vol. 10, pp. 885- 892 ,(1991) , 10.1002/J.1460-2075.1991.TB08021.X
Terry L. Delovitch, Isabelle Bergerot, Konstantin V. Salojin, Jian-Xin Gao, Mark J. Cameron, Jian Zhang, p38 Mitogen-Activated Protein Kinase Mediates Signal Integration of TCR/CD28 Costimulation in Primary Murine T Cells Journal of Immunology. ,vol. 162, pp. 3819- 3829 ,(1999)
M FELD, B DIMANT, A DELORENZI, O COSO, A ROMANO, Phosphorylation of extra-nuclear ERK/MAPK is required for long-term memory consolidation in the crab Chasmagnathus. Behavioural Brain Research. ,vol. 158, pp. 251- 261 ,(2005) , 10.1016/J.BBR.2004.09.005
Dominic C. H. Ng, Marie A. Bogoyevitch, The mechanism of heat shock activation of ERK mitogen-activated protein kinases in the interleukin 3-dependent ProB cell line BaF3. Journal of Biological Chemistry. ,vol. 275, pp. 40856- 40866 ,(2000) , 10.1074/JBC.M004639200
Gilles Pagès, Sandrine Guérin, Dominique Grall, Frédéric Bonino, Austin Smith, Fabienne Anjuere, Patrick Auberger, Jacques Pouysségur, Defective Thymocyte Maturation in p44 MAP Kinase (Erk 1) Knockout Mice Science. ,vol. 286, pp. 1374- 1377 ,(1999) , 10.1126/SCIENCE.286.5443.1374
Teri G. Boulton, Steven H. Nye, David J. Robbins, Nancy Y. Ip, Elizabeth Radzlejewska, Sharon D. Morgenbesser, Ronald A. DePinho, Nikos Panayotatos, Melanie H. Cobb, George D. Yancopoulos, ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF Cell. ,vol. 65, pp. 663- 675 ,(1991) , 10.1016/0092-8674(91)90098-J
Sheng Xiao, Jun Wang, Jianwei Jiang, Xiaoding Cao, Gencheng Wu, Hui Zhao, Characterization of Fyn signaling on the age-dependent immuno-modulation on traumatic rats. Brain Research. ,vol. 1255, pp. 162- 169 ,(2009) , 10.1016/J.BRAINRES.2008.12.007
Jianjie Chen, Jinling Cao, Yongju Luo, Lingtian Xie, Jing Song, Wenjuan Xue, Ruhui Jia, Jie Song, Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio) Acta Histochemica. ,vol. 116, pp. 1337- 1341 ,(2014) , 10.1016/J.ACTHIS.2014.08.005
Ekambaram Padmini, Jayachandran Tharani, Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes Environmental Monitoring and Assessment. ,vol. 187, pp. 4147- ,(2015) , 10.1007/S10661-014-4147-1
Joël Raingeaud, Shashi Gupta, Jeffrey S. Rogers, Martin Dickens, Jiahuai Han, Richard J. Ulevitch, Roger J. Davis, Pro-inflammatory Cytokines and Environmental Stress Cause p38 Mitogen-activated Protein Kinase Activation by Dual Phosphorylation on Tyrosine and Threonine Journal of Biological Chemistry. ,vol. 270, pp. 7420- 7426 ,(1995) , 10.1074/JBC.270.13.7420