Immunoneutralisation of GnRH-I, without cross-reactivity to GnRH-II, in the development of a highly specific anti-fertility vaccine for clinical and veterinary use ☆

摘要: In recent years, several forms of gonadotrophin releasing hormone (GnRH) molecules have been isolated from primate brain. These are very similar in sequence and this raises the question whether previously developed neutralisation vaccines based on GnRH (now termed GnRH-I) would remove other (namely GnRH-II) as well. As function these has not yet clearly defined, potential health risks could exist by their ablation. view high homology between molecules, paper describes production highly specific polyclonal antibodies against GnRH-I GnRH-II, with negligible cross-reactivity. The ultimate aim is to develop an anti-fertility vaccine which does present any inappropriate side-effects, caused a molecule may or be directly involved reproduction. Several formulations were investigated, analogues following conjugated tetanus toxoid: pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 GnRH-II pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2 specificity produced was examined, together effects fertility side-effects. Immunostaining hypothalamic sections carried out, using generated antisera, determine regional distribution neurones, well further evaluate specifity antibodies.