Crypt Cell Antigen Expression in Human Colon Tumor Cell Lines: Analysis With a Panel of Monoclonal Antibodies to CaCo-2 Luminal Membrane Components2

摘要: Twenty BALB/c mouse monoclonal antibodies to surface membrane components of confluent CaCo-2 cells were used study the expression small intestine markers in cultured human colon tumor cells. The antigens recognized by 15 these identified as proteins relatively high molecular weight, one them corresponding single-chain precursor sucrase-isomaltase. By immunofluorescence, 14 found stain epithelial cells, and various distinctive patterns antigen distribution jejunum observed. Five stained exclusively crypt 3 luminal membranes absorptive villus 4 entire epithelium. In contrast, only normal tissue. different defined this panel showed great variability their intestinal cell lines tested. These results demonstrated a surprising similarity between many set new that can be identify characterize vivo culture.