Expression of enzymatically active sucrase-isomaltase is a ubiquitous property of colon adenocarcinomas

摘要: Abstract Adenocarcinoma of the colon is one most prevalent and lethal all human malignancies. The early diagnosis management this disease could be improved if biological markers, whose expression was restricted to malignant cells, were identified. Sucrase-isomaltase a glycoprotein hydrolase expressed throughout small intestine fetal but not in normal adult colon. This study shows that enzymatically active sucrase-isomaltase ubiquitous property primary metastatic adenocarcinoma. Significant sucrase enzyme activity (i.e., > 5 mU/mg protein) observed 16 carcinomas adjacent mucosa. messenger RNA identified tumors using reverse transcriptase polymerase chain reaction. Using quantitative reaction analysis, amount examined (3.4 × 10 −8 3.19 −7 μg/μg total RNA) greater than mucosa (0 3.4 RNA). induction corroborated by immunostaining. Of 30 adenocarcinomas examined, 80% positive for sucrase-isomaltase. In addition, carcinoma metastases staining pattern distinct demarcated tumor cells from surrounding histologically tissue. No seen same patients. With exception lung, no immunostaining variety noncolonic adenocarcinomas. Thus, specificity ubiquity can exploited improve clinical disease. studies on structure gene its regulatory elements should contribute toward understanding alteration oncogenic transformation colonic