Interspecific Small Molecule Interactions between Clinical Isolates of Pseudomonas aeruginosa and Staphylococcus aureus from Adult Cystic Fibrosis Patients

摘要: Pseudomonas aeruginosa and Staphylococcus aureus are the most prevalent pathogens in airway infections of cystic fibrosis (CF) patients. We studied how these coexist interact with each other. Clinical isolates both species were retrieved from adult CF Culture supernatants 63 P. triggered a wide range biofilm-stimulatory activities when added to culture control S. strain. The extent biofilm formation by was positively correlated levels 2-alkyl-4-(1H)-quinolones (AQs) Quinolone Signal (PQS) 2-heptyl-4-hydroxy quinoline N-oxide (HQNO) produced isolates. Supernatants isogenic mutants deficient PQS HQNO production stimulated significantly less than that seen parental strain PA14. When studying co-isolated pairs patients showing pathogens, counterparts compared observed using Accordingly, some low exoproducts also clinical not their co-isolates or PA14 despite adequate HQNO. This suggests colonization lungs promotes type selection, co-existence requires specific adaptations either pathogens. Results provide insights on bacterial interactions CF.