Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil
作者： Vlademir Cantarelli , Bianca Cavalcante , Diogo André Pilger , Fabiana Souza , Cícero Gomes Dias
DOI: 10.1590/S0037-86822011000500021
关键词:
摘要: INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included a program. RESULTS: Sensitivity RT-PCR was similar primary culture (75% and 79.5%, respectively) when compared broth enriched culture, whereas specificity 83.1%. CONCLUSIONS: provides same day results, however it showed low sensitivity detection.
sci-hub.se 参考文章(9)
Walter J Hierholzer, Julia S Garner, Audrey B Adams, Donald E Craven, David W Fleming, Susan W Forlenza, M Gilchrist, D Goldmann, E Larson, C Mayhall, Rita D McCormick, Ronald L Nichols, CE Edmiston, DG Maki, G Pugliese, RA Weinstein, OC Tablan, FC Tenover, WJ Martone, RF Gaynes, WR Jarvis, MF Favero, Recommendations for preventing the spread of vancomycin resistance: Recommendations of the Hospital Infection Control Practices Advisory Committee (HICPAC) American Journal of Infection Control. ,vol. 23, pp. 87- 94 ,(1995) , 10.1016/0196-6553(95)90104-3
P. D. Stamper, M. Cai, C. Lema, K. Eskey, K. C. Carroll, Comparison of the BD GeneOhm VanR Assay to Culture for Identification of Vancomycin-Resistant Enterococci in Rectal and Stool Specimens Journal of Clinical Microbiology. ,vol. 45, pp. 3360- 3365 ,(2007) , 10.1128/JCM.01458-07
Douglas J. Biedenbach, Gary J. Moet, Ronald N. Jones, Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997–2002) Diagnostic Microbiology and Infectious Disease. ,vol. 50, pp. 59- 69 ,(2004) , 10.1016/J.DIAGMICROBIO.2004.05.003
Ricardo Titze-De-Almeida, Alex Van Belkum, Maria Sueli Soares Felipe, Rosemeire C. Zanella, Janetta Top, Rob J.L. Willems, Multilocus sequence typing of hospital-associated Enterococcus faecium from Brazil reveals their unique evolutionary history. Microbial Drug Resistance. ,vol. 12, pp. 121- 125 ,(2006) , 10.1089/MDR.2006.12.121
Pedro Alves d'Azevedo, Guilherme HC Furtado, Eduardo AS Medeiros, Kelly Aline Santiago, Suzane Silbert, Antonio CC Pignatari, None, Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil Revista Do Instituto De Medicina Tropical De Sao Paulo. ,vol. 50, pp. 195- 198 ,(2008) , 10.1590/S0036-46652008000400001
L. M. Sloan, J. R. Uhl, E. A. Vetter, C. D. Schleck, W. S. Harmsen, J. Manahan, R. L. Thompson, J. E. Rosenblatt, F. R. Cockerill, Comparison of the Roche LightCycler vanA/vanB Detection Assay and Culture for Detection of Vancomycin-Resistant Enterococci from Perianal Swabs Journal of Clinical Microbiology. ,vol. 42, pp. 2636- 2643 ,(2004) , 10.1128/JCM.42.6.2636-2643.2004
S. Palladino, I. D. Kay, J. P. Flexman, I. Boehm, A. M. G. Costa, E. J. Lambert, K. J. Christiansen, Rapid Detection of vanA and vanB Genes Directly from Clinical Specimens and Enrichment Broths by Real-Time Multiplex PCR Assay Journal of Clinical Microbiology. ,vol. 41, pp. 2483- 2486 ,(2003) , 10.1128/JCM.41.6.2483-2486.2003
I.L.B.C. Camargo, A.L. Barth, K. Pilger, B.G.S. Seligman, A.R.L. Machado, A.L.C. Darini, Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil Brazilian Journal of Medical and Biological Research. ,vol. 37, pp. 1669- 1671 ,(2004) , 10.1590/S0100-879X2004001100012
S. J. Drews, G. Johnson, F. Gharabaghi, M. Roscoe, A. Matlow, R. Tellier, S. E. Richardson, A 24-Hour Screening Protocol for Identification of Vancomycin-Resistant Enterococcus faecium Journal of Clinical Microbiology. ,vol. 44, pp. 1578- 1580 ,(2006) , 10.1128/JCM.44.4.1578-1580.2006