Hexokinase of calf trabecular meshwork.

摘要: Although aqueous outflow is most likely a passive, nonenergy-dependent process, alterations in cellular function the trabecular meshwork presumably are involved development of some types glaucoma. Accordingly, it seems important to define both normal and abnormal biochemistry this tissue. The authors have chosen glycolysis as their starting point, concentrating on regulatory enzymes, hexokinase, and, companion paper, phosphofructokinase. Hexokinase activity has been measured 100,000 X g supernatant homogenates prepared from excised calf meshwork. Treatment homogenate with Triton X-100 before centrifuging caused twofold increase measurable activity. Electrophoresis cellulose acetate revealed I II isoenzymes. starch gel further resolved type 1 into adult fetal subtypes. principal isoenzyme released solution by was 1. kinetic behavior hexokinase varying concentrations glucose at saturating levels ATP. Kms calculated these plots were 7.15 10(-2) M, 1.78 10(-3) 1.19 10(-4) M. Apart form isoenzymes resemble those other ocular tissues extraocular tissues. special role, if any, regulating not known. Possibly, "kinetically adaptable" isoenzyme.