Superoxide dismutase and catalase of calf trabecular meshwork.

摘要: Superoxide dismutase and catalase activities have been measured in cell-free extracts of calf trabecular meshwork, for comparison, iris, retina, lens, liver, erythrocytes. Gel electrophoresis has used to identify isozymes each enzyme. The superoxide per milligram wet weight 0.184 0.884 U/mg wt, respectively, were comparable those found iris much higher than lens. Three identified meshwork. Two these presumably correspond cytoplasmic dismutase, while the third corresponds a mitochondrial isozyme. A mitochondria! activity was also observed but not single isozyme all tissues examined. At physiologic H2O2 concentrations, may similar levels glutathione peroxidase. catalase, peroxidase constitute an important defense mechanism meshwork against toxic Ol which it must be continuously exposed from endogenous production as well aqueous humor. Invest Ophthalmol Vis Sci 26:1330-1335, 1985 (TM), forms major route outflow anterior chamber eye, shown metabolically active, respiring tissue calf. 1 cells TM, therefore, would expected possess mechanisms byproducts oxidative metabolism, such radical (O2) H2O2. In addition, TM probably need equipped withstand extracellular exposure same oxidants humor: is normal constituent humor many species, 2 light entering eye result vivo photochemical generation OI 3 Since O2 are apparently