Lateral spatial assay of zinc and superoxide dismutase in the ocular lens.

摘要: A procedure is described, using a freezing microtome and measurements of tissue slice volume, to obtain serial sections the lens for analysis components from one side organ other. The concentrations zinc (analyzed by atomic absorption spectrophotomctry) were similar in taken across anterior posterior surface. levels metallocnzyme supcroxide dismutase were, however, much higher sections. This quite simple may be useful analyzing distribution lenticular components. Invest Ophthalmol Vis Sci 31:153-155, 1990 ocular spheroidal avascular covered on its surface with layer epithelial cells. These cells are major site primary exchanges many metabolites minerals bathing aqueous humor. remaining bulk composed fiber different ages, integrity which confers transparency lens, but have less metabolic activity. Minerals such as sodium potassium, glucose aminoacids primarily enter side, 1 possibly leading presence gradients activity organ. types cells, their varying also contribute differences chemical composition. lateral measured following sectioning organ, example slicing it microtome. Such pieces only weigh few milligrams, determination unit weight these presents considerable technical difficulty due thawing condensation and/or evaporation water. Zinc an essential trace element that present lens. 2 ' 3 It associated enzymes, including two notable ones carbonic anhydrase superoxide dismutase. 4 - 5 We described estimating slices used measure latter enzyme rabbit